Microfluidic Techniques: Reviews and ProtocolsShelley D. Minteer Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. |
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acid Agarose gel amplification AmpliTaq Anal analysis annealing antibody approx avidin bake biofuel bonding buffer caffeine calcium capillary cardiomyocyte cell retention structure chamber Chem chemical concentration cycles detection dye-doped electrode electrophoresis enzyme epoxy etching fabrication flow fluorescence Fluorescent probe genomic glass plates hybridization hydrogels immobilization immunoassays Incubate integrated lab-on-a-chip laser ablation layer mask microchannels microchip Microfabricated Microfabricated chip microfluidic microfluidic channels microfluidic chip microfluidic devices microfluidic systems Microfluidic Techniques micromachining microreactor microscope miniaturized mold Molecular Biology molecules MSCs nanoparticles Note on-chip optical oxide pattern PDMS PDMS substrates performed Permalloy Petri dish photolithography photoresist plasma plastic polydimethylsiloxane polymer polymerase chain reaction protein pump reactor reagents remove reservoir Reviews and Protocols Rinse room temperature sample silane silica silica nanoparticles silicon soft lithography solution sperm step surface syringe target DNA template thermocyclers tion tubing U/µL vacuum wafer wafer frame wavelength