Transgenic Crops of the World: Essential Protocols

Front Cover
Ian S. Curtis
Springer Science & Business Media, Nov 30, 2004 - Technology & Engineering - 454 pages
Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.
 

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Contents

Transgenic rice plants
3
Transformation of wheat by biolistics
19
Genetic transformation of barley Hordeum Vulgare L by coculture of immature embryos with Agrobacterium
35
Maize transformation
45
Genetic Engineering of Oat Avena Sativa L Via the Biolistic Bombarments of Shoot Apical Meristems
63
Generation of Transgenic Rye
79
Particle inflow gunmediated Transformation of Sorghum Biocolor
89
Sugarcane Transformation
103
Agrobacteriummediated genetic transformation of cotton
243
Agrobacteriummediated transformation of potato
257
Genetic transformation of radish Raphanus sativus L by floraldipping
271
Genetic transformation of Allium cepa mediated by Agrobacterium Tumefaciens
281
Transformation of carrot
291
Production of transgenic cassava Manihot esculenta Crantz
301
Soybean transformation using the Agrobacteriummediated cotyledonarynode method
323
In vitro regeneration and transformation of Vicia Faba
337

Biolistic Transformation of Fescues and Ryegrasses
115
Transformation of Banana Using Microprojectile Bombardment
131
AgrobacteriumMediated Transformation of Citrus
145
Coffea SPP Genetic Transformation
159
Genetic Transformation of Tea
171
MicroprojectileMediated Transformation of Pineapple
187
Regeneration and Genetic Transformation of Apple Malus Spp
199
Genetic Transformation of Pear Via Agrobacteriummediated gene transfer
217
AgrobacteriumMediated Transformation of Grape Embryogenic Calli
229
Gene technology in pea
351
Agrobacteriummediated transformation of cabbage
361
Agrobacteriummediated transformation of canola
379
Transformation of cauliflower
389
Tomato transformationthe nuclear and chloroplast genomes
405
Genetic transformation of watermelon
425
Genetic transformation of sunflower
435
Abbreviations
453
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