Carleton's Histological Technique

70 per cent absolute alcohol acetic acid alum aqueous Bact block blood bone buffer calcium celloidin cells cellulose nitrate clear in xylene colour containing Counterstain coverslip cryostat cutting cytoplasm decalcification Dehydrate demonstration differentiation Dissolve distilled water dyes embedding medium emulsion enzyme examination eyepiece fibres film filter fixation fixed fluid fluorescence formal-saline formalin fresh frozen sections gelatine glycerin glycogen granules haematoxylin histological hydrochloric acid impregnation injection iodine knife LENDRUM light lipids material methyl methylene blue microscope microtome minutes mixture mordant mucins myelin nuclei object osmium tetroxide oxidation paraffin wax Path Pearse phosphotungstic acid picric acid pigment potassium preparation protozoa reaction reagent removed RESULTS Rinse in distilled running water sections to water slide smears sodium soluble specimen Stain Technol stained sections staining methods substances suitable synthetic resin medium Take sections tap water TECHNIQUE thick thin tissue usually Wash in running xylene xylene and mount